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Tanulmányok

Performance and comparison of CASA systems equipped with different phase-contrast optics

Author(s): 
Yeung CH, Nieschlag E.
Institute(s): 
Institute of Reproductive Medicine of the University, Münster, Germany

Abstract

Most computer-aided sperm analysis (CASA) systems for human spermatozoa use positive phase-contrast optics. In this study the performance of one of these (HTM) and two other systems using negative phase optics (IVOS and SM) were assessed by scrutinizing the video-playback of the analyzed images for errors in the recognition of motile and immotile spermatozoa and in the tracking of motile cells.

Development of a novel CASA system based on open source software for characterization of zebrafish sperm motility parameters

Author(s): 
Jonas G. Wilson-Leedy, Rolf L. Ingermann
Institute(s): 
Department of Biological Sciences and Center for Reproductive Biology, University of Idaho, Moscow

Abstract

Although computer-assisted sperm analysis (CASA) outperforms manual techniques, many investigators rely on non-automated analysis due to the high cost of commercial options. In this study, we have written and validated a free CASA software primarily for
analysis of fish sperm.

Computer-assisted sperm analysis (CASA) as a tool for monitoring sperm quality in fish

Author(s): 
D.E. Kime, K.J.W. Van Look, B.G. McAllister, G. Huyskens, E. Rurangwab, F. Ollevier
Institute(s): 
Department of Animal and Plant Sciences, The University of Sheffield, Sheffield S10 2TN, UK - Laboratory of Aquatic Ecology, Katholieke Universiteit Leuwen, Ch. De Beriotstraat 32, B-3000 Leuwen, Belgium

Abstract


The use of motility as a measure of sperm quality in fish is reviewed. Computer assisted sperm analysis (CASA) provides a simple and rapid quantitative assessment of the quality of fish sperm and may predict its ability to fertilise eggs.