Most computer-aided sperm analysis (CASA) systems for human spermatozoa use positive phase-contrast optics. In this study the performance of one of these (HTM) and two other systems using negative phase optics (IVOS and SM) were assessed by scrutinizing the video-playback of the analyzed images for errors in the recognition of motile and immotile spermatozoa and in the tracking of motile cells.
Although computer-assisted sperm analysis (CASA) outperforms manual techniques, many investigators rely on non-automated analysis due to the high cost of commercial options. In this study, we have written and validated a free CASA software primarily for analysis of fish sperm.
The use of motility as a measure of sperm quality in fish is reviewed. Computer assisted sperm analysis (CASA) provides a simple and rapid quantitative assessment of the quality of fish sperm and may predict its ability to fertilise eggs.
