Studies

Performance and comparison of CASA systems equipped with different phase-contrast optics

Abstract

Most computer-aided sperm analysis (CASA) systems for human spermatozoa use positive phase-contrast optics. In this study the performance of one of these (HTM) and two other systems using negative phase optics (IVOS and SM) were assessed by scrutinizing the video-playback of the analyzed images for errors in the recognition of motile and immotile spermatozoa and in the tracking of motile cells.

Development of a novel CASA system based on open source software for characterization of zebrafish sperm motility parameters

Abstract

Although computer-assisted sperm analysis (CASA) outperforms manual techniques, many investigators rely on non-automated analysis due to the high cost of commercial options. In this study, we have written and validated a free CASA software primarily for
analysis of fish sperm.

Computer-assisted sperm analysis (CASA) as a tool for monitoring sperm quality in fish

Abstract


The use of motility as a measure of sperm quality in fish is reviewed. Computer assisted sperm analysis (CASA) provides a simple and rapid quantitative assessment of the quality of fish sperm and may predict its ability to fertilise eggs.

Computer-assisted sperm analysis (CASA) as a tool for monitoring sperm quality in fish

Author(s): 
D.E. Kime, K.J.W. Van Look, B.G. McAllister, G. Huyskens, E. Rurangwab, F. Ollevier
Institute(s): 
Department of Animal and Plant Sciences, The University of Sheffield, Sheffield S10 2TN, UK - Laboratory of Aquatic Ecology, Katholieke Universiteit Leuwen, Ch. De Beriotstraat 32, B-3000 Leuwen, Belgium

Abstract


The use of motility as a measure of sperm quality in fish is reviewed. Computer assisted sperm analysis (CASA) provides a simple and rapid quantitative assessment of the quality of fish sperm and may predict its ability to fertilise eggs.

It has been used to: monitor the effects of heavy metal pollutants, such as mercury and tributyltin, on sperm quality; to select broodstock; to improve the efficiency of cryopreservation and storage; and to optimise conditions for fertilisation. In combination with CASA, morphological measurements can be used to determine the causes of reduced sperm motility. Technical details for the use of CASA are described.

 

2001 Elsevier Science Inc. All rights reserved.

Keywords: Sperm; Fish; Motility; Fertilisation rate; Computer assisted sperm analysis CASA.; Pollution; Crypreservation


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