Most computer-aided sperm analysis (CASA) systems for human spermatozoa use positive phase-contrast optics. In this study the performance of one of these (HTM) and two other systems using negative phase optics (IVOS and SM) were assessed by scrutinizing the video-playback of the analyzed images for errors in the recognition of motile and immotile spermatozoa and in the tracking of motile cells.
Although computer-assisted sperm analysis (CASA) outperforms manual techniques, many investigators rely on non-automated analysis due to the high cost of commercial options. In this study, we have written and validated a free CASA software primarily for analysis of fish sperm.
The use of motility as a measure of sperm quality in fish is reviewed. Computer assisted sperm analysis (CASA) provides a simple and rapid quantitative assessment of the quality of fish sperm and may predict its ability to fertilise eggs.
Gallery
Biologic sample
The most important data of the biologic sample is stored in the MiBio database. Each sample is defined by its own set of meta data and the images and videos uploaded.
Sample search
Search for samples any time later to carry out further tests. Multiple examinations on the same sample are supported.
Automatic analysis
Analysis is done automatically based on the previously selected settings. Available examinations: motility and viability tests, cell concentration test, morphological and DNA fragmentation tests.
Analytics settings
Manage the analytics settings for each of your examinations individually. Each examination type and microscope technique can have its own particular settings.
Knowledge base
Knowledge bases form the basis of morphological analysis carried out by the system. The more images, the better the qualification the better the results of your morphological analysis.
Trim and classify
Qualify images for your own species-specific knowledge bases. Each knowledge base may consist of several thousands of images.